BioTechnology

BioTechnology

Agriculture refers to the production of food, feed, fiber and other goods by the systematic growing of plants, animals and other life forms. The broad field of agricultural research is called agricultural science. Agriculture encompasses many subjects, including aquaculture, cultivation, animal husbandry, and horticulture. Each of these subjects can be further partitioned: for example, cultivation includes both organic farming and intensive farming, and animal husbandry includes ranching, herding, and intensive pig farming. Agricultural products include fodder, (starch, sugar, alcohols and resins), fibers (cotton, wool, hemp, silk and flax), fuels (methane from biomass, ethanol, biodiesel), cut flowers, ornamental and nursery plants, tropical fish and birds for the pet trade, and both legal and illegal drugs (biopharmaceuticals, tobacco, marijuana, opium, cocaine).

As of 2006, an estimated 36 percent of the world's workers are employed in agriculture[1] (down from 42% in 1996), making it by far the most common occupation. However, the relative significance of farming has dropped steadily since the beginning of industrialization, and in 2006 – for the first time in history – the services sector overtook agriculture as the economic sector employing the most people worldwide. Also, agricultural production accounts for less than five percent of the gross world product (an aggregate of all gross domestic products).

 
Agriculture played a key role in the development of human civilization—it is widely believed that the domestication of plants and animals allowed humans to settle and give up their previous hunter-gatherer lifestyle during the Neolithic Revolution. Until the Industrial Revolution, the vast majority of the human population labored in agriculture. Development of agricultural techniques has steadily increased agricultural productivity, and the widespread diffusion of these techniques during a time period is often called an agricultural revolution. A remarkable shift in agricultural practices has occurred over the past century in response to new technologies. In particular, the Haber-Bosch method for synthesizing ammonium nitrate made the traditional practice of recycling nutrients with crop rotation and animal manure less necessary. Synthetic nitrogen, along with mined rock phosphate, pesticides and mechanization, have greatly increased crop yields in the early 20th century. Increased supply of grains has lead to cheaper livestock as well. Further, global yield increases were experienced later in the 20th century when high-yield varieties of common staple grains such as rice, wheat, and corn were introduced as a part of the Green Revolution. The Green Revolution exported the technologies (including pesticides and synthetic nitrogen) of the developed world out to the developing world. Thomas Malthus famously predicted that the Earth would not be able to support its growing population, but technologies such as the Green Revolution have allowed the world to produce a surplus of food.

Many governments have subsidized agriculture to ensure an adequate food supply. These agricultural subsidies are often linked to the production of certain commodities such as wheat, corn, rice, soybeans, and milk. These subsidies, especially when done by developed countries have been noted as protectionist, inefficient, and environmentally damaging.In the past century agriculture has been characterized by enhanced productivity, the use of synthetic fertilizers and pesticides, selective breeding, mechanization, water contamination, and farm subsidies. Proponents of organic farming such as Sir Albert Howard argued in the early 1900s that the overuse of pesticides and synthetic fertilizers damages the long-term fertility of the soil. While this feeling lay dormant for decades, as environmental awareness has increased recently there has been a movement towards sustainable agriculture by some farmers, consumers, and policymakers. In recent years there has been a backlash against perceived external environmental effects of mainstream agriculture, particularly regarding water pollution, resulting in the organic movement. One of the major forces behind this movement has been the European Union, which first certified organic food in 1991 and began reform of its Common Agricultural Policy (CAP) in 2005 to phase out commodity-linked farm subsidies, also known as decoupling. The growth of organic farming has renewed research in alternative technologies such as integrated pest management and selective breeding. Recent mainstream technological developments include genetically modified food.

 

DNA Details

Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information and DNA is often compared to a set of blueprints, since it contains the instructions needed to construct other components of cells, such as proteins and RNA molecules. The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information.

Chemically, DNA is a long polymer of simple units called nucleotides, with a backbone made of sugars and phosphate groups joined by ester bonds. Attached to each sugar is one of four types of molecules called bases. It is the sequence of these four bases along the backbone that encodes information. This information is read using the genetic code, which specifies the sequence of the amino acids within proteins. The code is read by copying stretches of DNA into the related nucleic acid RNA, in a process called transcription. Most of these RNA molecules are used to synthesize proteins, but others are used directly in structures such as ribosomes and spliceosomes. Within cells, DNA is organized into structures called chromosomes. These chromosomes are duplicated before cells divide, in a process called DNA replication. Eukaryotic organisms such as animals, plants, and fungi store their DNA inside the cell nucleus, while in prokaryotes such as bacteria it is found in the cell's cytoplasm. Within the chromosomes, chromatin proteins such as histones compact and organize DNA, which helps control its interactions with other proteins and thereby control which genes are transcribed.

DNA is a long polymer made from repeating units called nucleotides.The DNA chain is 22 to 26 Ångströms wide (2.2 to 2.6 nanometres), and one nucleotide unit is 3.3 Ångstroms (0.33 nanometres) long. Although each individual repeating unit is very small, DNA polymers can be enormous molecules containing millions of nucleotides. For instance, the largest human chromosome, chromosome number 1, is 220 million base pairs long. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly-associated pair of molecules. These two long strands entwine like vines, in the shape of a double helix. The nucleotide repeats contain both the segment of the backbone of the molecule, which holds the chain together, and a base, which interacts with the other DNA strand in the helix. In general, a base linked to a sugar is called a nucleoside and a base linked to a sugar and one or more phosphate groups is called a nucleotide. If multiple nucleotides are linked together, as in DNA, this polymer is referred to as a polynucleotide. The backbone of the DNA strand is made from alternating phosphate and sugar residues. The sugar in DNA is 2-deoxyribose, which is a pentose (five carbon) sugar. The sugars are joined together by phosphate groups that form phosphodiester bonds between the third and fifth carbon atoms of adjacent sugar rings. These asymmetric bonds mean a strand of DNA has a direction. In a double helix the direction of the nucleotides in one strand is opposite to their direction in the other strand. This arrangement of DNA strands is called antiparallel. The asymmetric ends of DNA strands are referred to as the 5′ (five prime) and 3′ (three prime) ends. One of the major differences between DNA and RNA is the sugar, with 2-deoxyribose being replaced by the alternative pentose sugar ribose in RNA.

 

hydroponics,

Animals, including horses, mules, oxen, camels, llamas, alpacas, and dogs, are often used to help cultivate fields, harvest crops, wrangle other animals, and transport farm products to buyers. Animal husbandry not only refers to the breeding and raising of animals for meat or to harvest animal products (like milk, eggs, or wool) on a continual basis, but also to the breeding and care of species for work and companionship.Airplanes, helicopters, trucks, tractors, and combines are used in Western (and, increasingly, Eastern) agriculture for seeding, spraying operations for insect and disease control, harvesting, aerial topdressing and transporting perishable products. Radio and television disseminate vital weather reports and other information such as market reports that concern farmers. Computers have become an essential tool for farm management. In recent years, some aspects of intensive industrial agriculture have been the subject of increasing debate. The widening sphere of influence held by large seed and chemical companies, meat packers and food processors has been a source of concern both within the farming community and for the general public. Another issue is the type of feed given to some animals that can cause bovine spongiform encephalopathy in cattle. There has also been concern over the effect of intensive agriculture on the environment.

The patent protection given to companies that develop new types of seed using genetic engineering has allowed seed to be licensed to farmers in much the same way that computer software is licensed to users. This has changed the balance of power in favor of the seed companies, allowing them to dictate terms and conditions previously unheard of. The Indian activist and scientist Vandana Shiva argues that these companies are guilty of biopiracy.Soil conservation and nutrient management have been important concerns since the 1950s, with the most advanced farmers taking a stewardship role with the land they use. However, increasing contamination of waterways and wetlands by nutrients like nitrogen and phosphorus are concerns that can only be addressed by "enlightenment" of farmers and/or far stricter law enforcement in many countries.Increasing consumer awareness of agricultural issues has led to the rise of community-supported agriculturecal food movement, "Slow Food", and commercial organic farming.

 

Ancient origins

Developed independently by geographically distant populations, systematic agriculture first appeared in Southwest Asia in the Fertile Crescent, particularly in modern-day Iraq and Syria/Israel. Around 9500 BCE, proto-farmers began to select and cultivate food plants with desired characteristics. Though there is evidence of earlier sporadic use of wild cereals, it was not until after 9500 BCE that the eight so-called founder crops of agriculture appear: first emmer and einkorn wheat, then hulled barley, peas, lentils, bitter vetch, chick peas and flax.By 7000 BCE, small-scale agriculture reached Egypt. From at least 7000 BCE the Indian subcontinent saw farming of wheat and barley, as attested by archaeological excavation at Mehrgarh in Balochistan. By 6000 BCE, mid-scale farming was entrenched on the banks of the Nile. About this time, agriculture was developed independently in the Far East, with rice, rather than wheat, as the primary crop. Chinese and Indonesian farmers went on to domesticate mung, soy, azuki and taro. To complement these new sources of carbohydrates, highly organized net fishing of rivers, lakes and ocean shores in these areas brought in great volumes of essential protein. Collectively, these new methods of farming and fishing inaugurated a human population boom dwarfing all previous expansions, and is one that continues today.

By 5000 BCE, the Sumerians had developed core agricultural techniques including large scale intensive cultivation of land, mono-cropping, organized irrigation, and use of a specialized labour force, particularly along the waterway now known as the Shatt al-Arab, from its Persian Gulf delta to the confluence of the Tigris and Euphrates. Domestication of wild aurochs and mouflon into cattle and sheep, respectively, ushered in the large-scale use of animals for food/fiber and as beasts of burden. The shepherd joined the farmer as an essential provider for sedentary and semi-nomadic societies.Maize, manioc, and arrowroot were first domesticated in the Americas as far back as 5200 BCE. The potato, tomato, pepper, squash, several varieties of bean, Canna, tobacco and several other plants were also developed in the New World, as was extensive terracing of steep hillsides in much of Andean South America.In later years, the Greeks and Romans built on techniques pioneered by the Sumerians but made few fundamentally new advances. The Greeks and Macedonians struggled with very poor soils, yet managed to become dominant societies for years. The Romans were noted for an emphasis on the cultivation of crops for trade.

 

RNA Information

Ribonucleic acid or RNA is a nucleic acid, consisting of many nucleotides that form a polymer. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA plays several important roles in the processes of translating genetic information from deoxyribonucleic acid (DNA) into proteins. One type of RNA acts as a messenger between DNA and the protein synthesis complexes known as ribosomes, others form vital portions of the structure of ribosomes, act as essential carrier molecules for amino acids to be used in protein synthesis, or change which genes are active. RNA is very similar to DNA, but differs in a few important structural details: RNA is usually single stranded, while DNA is usually double stranded. RNA nucleotides contain ribose while DNA contains deoxyribose (a type of ribose that lacks one oxygen atom), and RNA uses the nucleotide uracil in its composition, instead of thymine which is present in DNA. RNA is transcribed from DNA by enzymes called RNA polymerases and is generally further processed by other enzymes, some of them guided by non-coding RNAs.

RNA is a polymer with a ribose and phosphate backbone and four different nucleotide bases: adenine, guanine, cytosine, and uracil. The first three are the same as those found in DNA, but in RNA thymine is replaced by uracil as the base complementary to adenine. This base is also a pyrimidine and is very similar to thymine. In DNA, however, uracil is readily produced by chemical degradation of cytosine, so having thymine as the normal base makes detection and repair of such incipient mutations more efficient. Thus, uracil is appropriate for RNA, where quantity is important but lifespan is not, whereas thymine is appropriate for DNA where maintaining sequence with high fidelity is more critical. There are also numerous modified bases and sugars found in RNA that serve many different roles. Pseudouridine (Ψ), in which the linkage between uracil and ribose is changed from a C–N bond to a C–C bond, and ribothymidine (T), are found in various places (most notably in the TΨC loop of tRNA). Another notable modified base is hypoxanthine (a deaminated guanine base whose nucleoside is called inosine). Inosine plays a key role in the Wobble Hypothesis of the genetic code. There are nearly 100 other naturally occurring modified nucleosides, of which pseudouridine and nucleosides with 2'-O-methylribose are by far the most common. The specific roles of many of these modifications in RNA are not fully understood. However, it is notable that in ribosomal RNA, many of the post-translational modifications occur in highly functional regions, such as the peptidyl transferase center and the subunit interface, implying that they are important for normal function.

The most important structural feature of RNA, that distinguishes it from DNA is the presence of a hydroxyl group at the 2'-position of the ribose sugar. The presence of this functional group enforces the C3'-endo sugar conformation (as opposed to the C2'-endo conformation of the deoxyribose sugar in DNA) that causes the helix to adopt the A-form geometry rather than the B-form most commonly observed in DNA. This results in a very deep and narrow major groove and a shallow and wide minor groove. A second consequence of the presence of the 2'-hydroxyl group is that in conformationally flexible regions of an RNA molecule (that is, not involved in formation of a double helix), it can chemically attack the adjacent phosphodiester bond to cleave the backbone.

 

Comparison with DNA

RNA and DNA differ in three main ways. First, unlike DNA which is double-stranded, RNA is a single-stranded molecule in most of its biological roles and has a much shorter chain of nucleotides. Secondly, while DNA contains deoxyribose, RNA contains ribose, (there is no hydroxyl group attached to the pentose ring in the 2' position in DNA, whereas RNA has two hydroxyl groups). These hydroxyl groups make RNA less stable than DNA because it is more prone to hydrolysis. Thirdly, the complementary nucleotide to adenine is not thymine, as it is in DNA, but rather uracil, which is an unmethylated form of thymine. Like DNA, most biologically active RNAs including tRNA, rRNA, snRNAs and other non-coding RNAs (such as the SRP RNAs) are extensively base paired to form double stranded helices. Structural analysis of these RNAs have revealed that they are not, "single-stranded" but rather highly structured. Unlike DNA, this structure is not just limited to long double-stranded helices but rather collections of short helices packed together into structures akin to proteins. In this fashion, RNAs can achieve chemical catalysis, like enzymes. For instance, determination of the structure of the ribosome — an enzyme that catalyzes peptide bond formation — revealed that its active site is composed entirely of RNA.

Synthesis of RNA is usually catalyzed by an enzyme - RNA polymerase, using DNA as a template. Initiation of synthesis begins with the binding of the enzyme to a promoter sequence in the DNA (usually found "upstream" of a gene). The DNA double helix is unwound by the helicase activity of the enzyme. The enzyme then progresses along the template strand in the 3’ -> 5’ direction, synthesizing a complementary RNA molecule with elongation occurring in the 5’ -> 3’ direction. The DNA sequence also dictates where termination of RNA synthesis will occur. There are also a number of RNA-dependent RNA polymerases as well that use RNA as their template for synthesis of a new strand of RNA. For instance, a number of RNA viruses (such as poliovirus) use this type of enzyme to replicate their genetic material. Also, it is known that RNA-dependent RNA polymerases are required for the RNA interference pathway in many organisms

 

History Of Conservation ethic

The origins of biological conservation can be traced to philosophical and religious beliefs about Man as a full part of Nature. Conserving natural resources and the environment is not a recent concern, but has deep cultural roots and the protection of nature, and especially forests, has been promoted for centuries.The Torah, or Old Testament discusses the concept of the Sabbatical Year, a period whereby the fields are left fallow, presumably in order to rejuvenate the soil. This would appear to be an ancient form of the ecological practice of crop rotation. The weekly Sabbath is also a time when beasts of burden are given rest from their work. The Torah further prohibits the destruction of fruit bearing trees, and this commandment has been extended to encompass all manner of wastefulness.Taoist and Shintoist philosophies encourage recognition of special sites, allowing spiritual experiments.

Jainism, Hinduism and Buddhism grant a sacred value to animals. Primitive religions also recognize sacred values to sites such as forests, lakes, mountains. Islam recognizes each species as its own "nation", and an obligation of man to khalifa, or "stewardship" of the Earth. Specific conservation mechanisms such as haram and hima zones, and the origins of the idea of carrying capacity, were a product of Islamic civilization. Indigenous strategies successfully combated soil erosion and deforestation in pre-colonial East Africa, as well as in the early colonial empires in China and Venice. As early as 450 BCE Artaxerxes I attempted to restrict cutting Lebanese timber (Grove 1992). Plato, writing in the 4th century BCE, noted that the removal of trees in Attica produced soil erosion "and what remains is like the skeleton of a body wasted by disease". Some historians claim that the idea of conservation originated in conflicts over the use of forests (Glacken 1965).